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. 2022 Nov 3;46(1):28–42. doi: 10.1002/jimd.12570

FIGURE 2.

FIGURE 2

BBP‐671 renders PANK resistant to C3‐CoA inhibition in vitro. (A) Schematic diagram illustrating BBP‐671 mechanism of action. PANKs exist in two conformations: a catalytically inactive conformation that is stabilized by acyl‐CoA binding and a catalytically competent state that is stabilized by ATP binding. BBP‐671 binding stabilizes the active conformation by binding across the dimer interface and preventing acetyl‐CoA binding and inhibition. (B) Inhibition of PANK1β by C3‐CoA in the presence (red) and absence (black) of BBP‐671 as described in Methods. (C) Inhibition of PANK2 (the mature processed form of human PANK2) by C3‐CoA in the presence (red) and absence (black) of BBP‐671. (D) Inhibition of PANK3 by C3‐CoA in the presence (red) and absence (black) of BBP‐671. Data from four data sets represent the mean ± SD. Lines were fit using the variable slope equation with GraphPad software.