FIGURE 2.

Berberine plays a cardioprotective role by mediating the immune cells and cytokines in mice with Myocardial infarction (MI). (a) Flow cytometry gating strategies for each cell population. (b) The bar charts show the percentages of neutrophils, Macrophages, Ly6C^high macrophages, Ly6C^low macrophages, DCs, (c) and CD4⁺ T cells, Foxp3⁺CD4⁺ Treg cells, ROR‐γt⁺CD4⁺ T, and CD8⁺ T cells by flow cytometry. (d) The mRNA expression of TNF‐α, IL‐6, IL‐18, MIP‐1β, TGF‐β, IL‐10, VEGF‐α by RT‐PCR, using infarcted heart tissues from the sham group, MI group, and Berberine group. n = 6 animals per group. Data are presented as mean ± SEM. *p < .05, **p < .01, ***p < .001