FIGURE 3:
Ady4 is a targeting subunit for Mss4. (A) Wild-type (AN120) containing SPC42::TagBFP (pRS306-spc42c-TagBFP), sso1∆ SPC42::TagBFP (GND10), and sso1∆ ady4∆ SPC42::TagBFP (GND11) diploids were transformed with plasmids expressing GFP1-10 (pRS423-PTEF1-GFP1-10) and MSS4-5xGFP11 (pRS414-Mss4-5xGFP11). Images of cells in meiosis II are shown for each strain. Arrows indicate colocalization of Mss4-5xGFP with the Spc42 marker. Scale bars = 5 µm. (B) The frequency of colocalization of Mss4 and Spc42 was measured in the cultures shown in A. For sso1∆ SPC42::TagBFP (n = 358) and sso1∆ ady4∆ (n = 225), at least 50 meiosis II SPBs were scored in six and four independent experiments, respectively. Error bars indicate one SD.