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. 2023 Mar 14;34(4):ar33. doi: 10.1091/mbc.E22-11-0515

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© 2023 Neiman et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 7: — Positive charges in the Spo21 N terminus promote proper prospore membrane number. (A) WT (AN120), ady4∆ (AC18), spo21::GFP (AN230), and spo21-3A (GND4) cells were transformed with the prospore membrane marker TagBFP-SPO20⁵¹^–⁹¹. Cells were sporulated and the number of prospore membranes in individual cells were scored. At least 80 cells were scored in each of three independent experiments for each strain. (B) A maximum intensity projection of a WT cell with four prospore membranes and a spo21-3A cell displaying more than four prospore membranes. Scale bars = 2 µm.