TABLE 2:
Plasmids used in this study.
| Plasmids | Description | Source |
|---|---|---|
| pBTM116-MSS4 FL | lexA fused to the N terminus of Mss4 | This study |
| pBTM116-MSS4I | lexA fused to the N terminus of Mss4 1–446 | This study |
| pBTM116-MSS4II | lexA fused to the N terminus of Mss4 334–779 | This study |
| pBTM116-MSS4III | lexA fused to the N terminus of Mss4 379–556 | This study |
| pBTM116-MSS4IV | lexA fused to the N terminus of Mss4 557–756 | This study |
| pBTM116-MSS4M | lexA fused to the N terminus of Mss4 334–446 | This study |
| pBTM116-MSS4N∆ | lexA fused to the N terminus of Mss4 with the internal deletion 347–364∆ | This study |
| pFA6-KanMX6 | Template for knockout PCR | Longtine et al., 1998 |
| pGADGH-ADY4 | Full-length GAD fusion | Nickas et al., 2003 |
| pGADGH-VPS13I | GAD fused to the N-terminal 964 aa of Vps13 | This study |
| pRS306-spc42C | C-terminal GFP for tagging | This study |
| pRS306-Spo21 | Integrating WT SPO21 | This study |
| pRS306-Spo21-3AR55A,K59A and K66A | Integrating mutant SPO21 | This study |
| pRS306-sSpc42C-TagBFP | Spc42 C terminus fused to TagBFP | This study |
| pRS314-HTB1-GFP | Histone H2B fused to GFP | This study |
| pRS314-HTB1-mOrange | Histone H2B fused to mOrange2 | This study |
| pRS316-HTB1-mOrange | Histone H2B fused to mOrange2 | This study |
| pRS404-PYKL050c-CFP | Spore-specific reporter | Thacker et al., 2011 |
| pRS404-PYKL050c-TagBFP | TagBFP under spore autonomous promoter | This study |
| pRS414-Mss4 GFP | Single GFP tag | Ling et al., 2012 |
| pRS414-Mss4-5xGFP11 | Mss4 fused to five copies of GFP β strand 11 | This study |
| pRS414-Mss4347–364∆GFP | GFP-tagged internal deletion | Ling et al., 2012 |
| pRS415-Stt4 GFP | C-terminal GFP tag | Audhya and Emr, 2002 |
| pRS423-PTEF1-1-10GFP | β strands 1–10 of GFP | This study |
| pRS424-PTEF1-Pex3-yEGFP | Pex3 GFP fusion | This study |
| pRS425-Mss4 | High copy WT gene | Coluccio et al., 2004 |
| pRS425-Pik1 | High copy WT gene | This study |
| pRS425-PTEF1-1-10GFP | β strands 1–10 of GFP | Chen et al., 2020 |
| pRS426-PGAL1-5xGFP11 | Tandem copies of 11th β strand | Chen et al., 2020 |
| pRS426-PTEF1pRS426-GFP Spo14 | Constitutive promoterInternal GFP-tagged WT gene | Mumberg et al., 1995 Rudge et al., 2001 |
| pRS426-PTEF1-1-10GFP | β strands 1–10 of GFP | This study |
| pRS426-PTEF1-Ady4-mOrange Pex15 | Peroxisome-targeted Ady4-mOrange | This study |
| pRS426-PTEF1-GFP-Spo2149–66 | Amphipathic helix of Spo21 | This study |
| pRS426-PTEF1-GFP-Spo2149–66-3A | Mutant amphipathic helix of Spo21 | This study |
| pRS426-PTEF1-mOrange2 Pex15 | Peroxisome-targeted mOrange | This study |
| pRS426-PTEF1-Spo20-51–91TagBFP | Prospore membrane marker | Jin et al., 2017 |
| pRS426-Stt4 | High copy WT gene | This study |
| pUC57-mOrange2-ScOpt | Saccharomyces codon-optimized mOrange2 | This study |
| pUC57-TagBFP-ScOptYEP351-GFP SPO14 | Saccharomyces codon-optimized TagBFPHigh copy GFP-tagged WT gene | This studyRudge et al., 1998 |
| YEP351-LSB6 | High copy WT gene | Audhya and Emr, 2002 |