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. 2023 Mar 7;34(4):ar31. doi: 10.1091/mbc.E22-11-0498

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© 2023 Gaeta and Tyska. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 2: — KIAA1671 is an uncharacterized protein that targets the brush border cytoskeleton. (A) A paraffin-embedded mouse small-intestinal tissue section stained with anti-KIAA1671 (cyan) and anti-Villin (magenta) antibodies. Zoom 1 highlights apical staining on the villus, while Zoom 2 highlights apical staining in intestinal crypts. Scale bar = 50 µm. (B) Representative W4 cell expressing an EGFP-KIAA1671 construct (cyan), stained with an anti-EPS8 antibody (yellow) and phalloidin to mark F-actin (magenta). Zoom 1 highlights KIAA1671 at the base of the brush border, while Zoom 2 highlights KIAA1671 localization in the cytoplasm. (C) Linescans measuring anti-KIAA1671 (Left) or EGFP-KIAA1671 (Right) at the apical surfaces of intestinal tissue and W4 cells, respectively. n = 63 linescans of villus intestinal tissue from one independent staining; n = 40 line cans of W4 brush borders from three independent experiments. (D) Quantification of the fraction of overlap between KIAA1671 and F-actin. Bars indicate the fraction of total KIAA1671 signal that overlaps with F-actin signal. n = 40 cells from three independent experiments. All images are displayed as maximum-intensity projections.