FIGURE 5.

Retinoic acid affects the expression of WNT inhibitors. Palates were isolated from E13.5 mouse embryos from nine different mothers, cultured oral‐side up for 4 days with 0.5–2 μM or without retinoic acid and then processed for qPCR or fixed for histology. (A) Relative WNT inhibitors gene expression. Data are represented as the mean ±  SD (N  =  3). * p <   0.05, ** p <   0.01, *** p <   0.001. M: molar. OS: oral side. NC: nasal cavity (B) Immunohistochemistry of WNT inhibitors. Representative pictures of the staining of each group. Black arrows: protein expression. Scale bars: 200 μm