TABLE 1.
Primer sequences
| Gene category | Symbol | Forward Primer (5′−3′) | Reverse Primer (5′−3′) |
|---|---|---|---|
| RA RESPONSIVE | Cyp26b1 | GATCCTACTGGGCGAACACC | GGAGAAGACCTTGCGCTTGT |
| Crabp2 | TGATCTCGACTGCTGGCTTG | TCCCATCGGGTTCCCATAAAG | |
| Rarb | GAAAACGACGACCCAGCAAG | TTACACGTTCGGCACCTTTC | |
| APOPTOSIS MARKER | Trp53 | GGAAGACTCCAGTGGGAACC | CTTCTGTACGGCGGTCTCTC |
| PROLIFERATION MARKER | Pcna | AGAGCATGGACTCGTCTCA | CCAGCACATTTTAGAATTTTGGACA |
| WNT TARGET | Axin2 | GGTTCCGGCTATGTCTTTGC | CAGTGCGTCGCTGGATAACTC |
| WNT INHIBITORS | Dkk3 | GGCCCACAGTCTTCATCAAT | CCAGAGTGGACAGGTGGTCT |
| Dkk1 | CGGGGGATGGATATCCCAGAA | ACGGAGCCTTCTTGTCCTTTG | |
| Kremen1 | TGGGTTTCCATGATCCTTGT | GCATGAGGACGGAGTCTACTG | |
| Sfrp1 | TCTAAGCCCCAAGGTACAACC | GCTTGCACAGAGATGTTCAATG | |
| Sfrp4 | ATCATCCTTGAACGCCACTC | TCGAACACAAGTCCCTCTCA | |
| Wif1 | GCATTCTTTGTTGGGCTTTC | CCATCAGGCTAGAGTGCTCA | |
| BONE DIFFERENTIATION MARKERS | Alpl | CCAGCAAGAAGAAGCCTTTG | AACCCAGACACAAGCATTCC |
| Col1a2 | CCTGGCAAAGACGGACTCAAC | GCTGAAGTCATAACCGCCACTG | |
| Runx2 | CGGACGAGGCAAGAGTTTCA | GGATGAGGAATGCGCCCTAA |