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. 2023 Mar 13;19(6):1731–1747. doi: 10.7150/ijbs.78554

Figure 6.

Figure 6

Effect of CM728, or Prdx1 knockdown on MAPKs, STAT3 and Akt in TNBC cell lines. A, Time-dependent effect of CM728 (1 µM) on MAPKs, STAT3 and Akt phosphorylation in MDA-MB-231 (left) and BT-549 cells (right). B, Immunoblot of phosphorylated MAPKs, STAT3 and Akt in Prdx1-knocked down TNBC cells. Lysates from cells treated with CM728 (5 µM) for 3 h were used as a positive control. C, Analysis of MAPK and STAT3 phosphorylation in MDA-MB-231 cells that were preincubated with 2 mM NAC or 1000 U/ml catalase for 2 h and then treated with 1 µM CM728 for 12 h. D, Dimerization of STAT3 in response to CM728 (1 µM) (D: dimer; M: monomer). β-actin or calnexin was used as loading controls. Densitometry quantification from immunosignal values is shown below the bands. Phosphorylated forms of proteins are relativized to the total amount of the corresponding proteins. In all the cases values are relativized to the loading control (A, C). In red, band densitometry compared to shC. Representative images of two independent experiments are shown.