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Double knockout of Gαi1 and Gαi3 in mouse embryonic fibroblasts (MEFs) abolishes SCF-induced signaling. The listed MEFs were stimulated with SCF at indicated concentrations and cultured for indicated periods, and the listed signaling protein levels were examined (A-F). “Ctrl” refers to PBS treatment. *P < 0.05 versus “WT MEFs”. #P < 0.05 (F). “N. S.” denotes P > 0.05.
