Figure 4.

SCF-induced signaling and pro-angiogenic activity are inhibited in endothelial cells with Gαi1 and Gαi3 mutations. HUVECs, with the dominant negative (DN) mutant-Gαi1 (human) construct plus the DN Gαi3 (human) (“DN-Gαi1/3”), construct or the vector control (“Vec”), were treated with SCF (50 ng/mL) for 15 min, and expression of listed proteins is shown (A and B). HUVECs were further cultured, and cell proliferation (C) and in vitro migration (D) were tested. * P< 0.05 versus “Vec” cells. “N. S.” denotes P > 0.05. Scale bar = 100 μm.