Figure 5.

Gαi1 and Gαi3 overexpression strengthens SCF-induced signaling and pro-angiogenic activity in endothelial cells. HUVECs were transduced with the lentiviral human Gαi1-expressing construct plus the lentiviral human Gαi3-expressing vector, and two stable colonies, “oeGαi1/3-Slc1” and “oeGαi1/3-Slc2”, were obtained after selection. Control HUVECs were transduced with vector control (“Vec”). HUVECs were then treated with SCF (50 ng/mL) for 15 min and listed mRNA and protein levels were examined (A-C). HUVECs were further cultured, and cell proliferation (D), in vitro migration (E), and capillary tube formation (F) were tested. * P< 0.05 versus “Vec”. “N. S.” denotes P > 0.05.