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. 2023 Mar 21;19(6):1861–1874. doi: 10.7150/ijbs.82567

Figure 3.

Figure 3

TNF-α induces stemness of BLBC cells partially by activating KLF5-EphA2. (A-E) Knockdown of EphA2 and KLF5 in HCC1937 and HCC1806, respectively, with concomitant addition of TNF-α for WB assay of EphA2 and KLF5 expression (A), ALDH+ cell ratio by flow assay (B-C), Detection of the sphere-forming rate of HCC1937 by microsphere-formation assay (D-E), Scale bar=250 μm; (F-J) Knockdown of KLF5 and reversion to overexpression of EphA2 and addition of TNF-α in HCC1937 and HCC1806 for WB detection of EphA2 and KLF5 expression (F), ALDH+ cell ratio by flow assay (G-H), Detection of the sphere-forming rate of HCC1937 by mamosphere formation assay (I-J), Scale bar=250 μm. Changed folds in (A) and (F) of indicated proteins were referenced to Control (siNC or pCDH), normalized to GAPDH, respectively.