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. 2023 Mar 21;19(6):1861–1874. doi: 10.7150/ijbs.82567

Figure 4.

Figure 4

Inhibiting EphA2 phosphorylation by ALW-II-41-27 impedes BLBC cell stemness and chemoresistance. (A-B) Detection of sphere formation by mamosphere formation assay after treating HCC1937 cells with chemotherapeutic drugs PTX (2 μM) and DDP (2 μM) alone or in combination with ALW-II-41-27 (50 nM) for 48 h. Scale bar=250 μm. (C-D) Detection of the ALDH+ cell ratio by flow cytometry after treatment of HCC1937 and HCC1806 cells with the chemotherapeutic agents PTX (2 μM) or DDP (2 μM) alone or in combination with ALW-II-41-27 (50 nM) for 48 h; (E-F) Detection of EphA2 and EphA2 pS897 expression by WB after treatment of HCC1937 and HCC1806 cells with the chemotherapeutic agents PTX (2 μM) or DDP (2 μM) alone or in combination with ALW-II-41-27 (50 nM) for 48 h; (G-H) IC50 assay in HCC1937 and HCC1806 cells treated with chemotherapeutic agents PTX or DDP alone or in combination with ALW-II-41-27. Changed folds in (E) and (F) of indicated proteins were referenced to DMSO, normalized to GAPDH, respectively.