Table 1.
Primer sequence and antibody information.
| Primer | Sequence | Gene ID | |
|---|---|---|---|
| acta2 | F: GCATCCACGAAACCACCTA | NM_007392.3 | |
| R: CACGAGTAACAAATCAAAGC | |||
| col1a1 | F: CAATGGCACGGCTGTGTGCG | NM_007742.4 | |
| R: AGCACTCGCCCTCCCGTCTT | |||
| gapdh | F: AAGTTGTCATGGATGACC | NM_001289726.1 | |
| R: TCACCATCTTCCAGGAGC | |||
| lrat | F: CTGACCAATGACAAGGAACGCACTC | NM_023624.4 | |
| R: CTAATCCCAAGACAGCCGAAGCAAGAC | |||
| Map1lc3b | F: TTCTTCCTCCTGGTGAATGG | NM_026160.5 | |
| R: GTGGGTGCCTACGTTCTCAT | |||
| Mki67 |
F: AATCCAACTCAAGTAAACGGGG R: TTGGCTTGCTTCCATCCTCA |
NM_001081117.2 | |
| Antibody | Source | Dilution | |
| collagen I | Abcam, ab270993 | 1:200 | |
| cytokeratin 7 | Abcam, ab199718 | 1:500 | |
| desmin | Abcam, ab8592 | 1:300 | |
| lc-3b | Abcam, ab192890 | 1:200 | |
| α-sma | Abcam, ab15734 | 1:300 | |
| sqstm1/p62 | Abcam, ab91526 | 1:500 | |
| Goat Anti-Rabbit IgG H&L (Alexa Flour 488- conjugated) | Abcam, Ab150077 | 1:500 | |
| Goat anti-Rabbit IgG HRP-conjugated | Abcam, ab6721 | 1:500 | |