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. 2023 Apr 4;13(7):1349. doi: 10.3390/diagnostics13071349

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Evaluation of the ddPCR duplex assay (MPXV as Channel 1 and RPP30 as Channel 2) performance using MPXV synthetic amplicon Gblock Sequence as reference DNA. Standard curves were obtained from 10-fold serial dilutions of the reference DNA (3.5 × 10⁴ copies/µL) in four biological matrices in two different transport media (UTM and eSwab) (A–G). Positive samples (up) and negative samples (below) (H).