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. 2022 Nov 21;227(7):888–900. doi: 10.1093/infdis/jiac450

Figure 2.

Figure 2.

Assessment of BKPyV genome load, DNase protection, and amplicon length. BKPyV loads were retrospectively analyzed in longitudinal urine (n = 77) and plasma (n = 73) samples from 20 HCT patients by 3 QNATs with different amplicon size (88 bp, 133 bp, and 239 bp). DNase I sensitivity of urine and plasma BKPyV genome loads as well as of cell-free human genomic DNA was assessed by DNAse I digestion prior to nucleic acid extraction, followed by BKPyV and ACY QNAT. Statistical comparison of nonparametric data was done using Mann-Whitney U test. A, Impact of BKPyV DNA fragment size on retrospective urine and plasma BKPyV load quantification (median, 25th and 75th percentiles). B, DNase I sensitivity of urine BKPyV loads and cell-free human genomic DNA. C, DNase I sensitivity of plasma BKPyV loads and cell-free human genomic DNA. Abbreviations: ACY, aspartoacylase; BKPyV, BK polyomavirus; HCT, hematopoietic cell transplantation; QNAT, quantitative nucleic acid testing.