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. 2023 Mar 25;13(7):1158. doi: 10.3390/ani13071158

Figure 3.

Figure 3

Chromogenic immunohistochemistry of anti-AQP-7 antibody in head (EH), body (EB) and tail (ET) of epididymis. (A,E,I): negative control of anti-AQP-7 antibody in EH, EB and ET during rutting season; (C) intensely positive reaction of AQP-7 in EH during rutting season shows strong positivity in basal cell (stem cell) layer and sterocelia of the columnar cell; (G) positive reaction of AQP-7 in EB during rutting season shows intense positivity of sterocelia of the columnar cell and (K) positive reaction of AQP-7 in ET during rutting season shows intense positivity of columnar cell. (B,F,J): negative control of AQP-7 in EH, EB and ET during non-rutting season; (D,H,L) are positive reactions of AQP-7 in EH, EB and ET during non-rutting season, which shows very weak positivity in both the basal cell and columnar cell. AQP-7 is stained by DAB/chromogen and counter-stained with hematoxylin under magnification power 40×.