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. 2023 Mar 25;13(7):1158. doi: 10.3390/ani13071158

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© 2023 by the author.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Chromogenic immunohistochemistry of anti-AQP-7 antibody in the initial part (DI), middle part (DM) and ampullary part of ductus deferens (DA). (A,E,I): negative control of anti-AQP-7 antibody in DI, DM and DA during rutting season; (C,G,K): positive reaction of AQP-7 in DI, DM and DA of ductus deferens during rutting season shows very intense positivity of AQP-7. (B,F,J): negative control of AQP-7 in DI, DM and DA of ductus deferens during non-rutting season; (D,H): positive reaction of AQP-7 in DI and DM of ductus deferens during non-rutting season shows weak positivity of AQP-7. (L): positive reaction of AQP-7 in the ampullary part of ductus deferens during non-rutting season shows moderate positivity of the columnar cell. AQP-7 was stained by DAB/chromogen and counter-stained with hematoxylin under magnification power 40×.