Figure 4.

Protein modeling of CYB5R3. (A) Protein model of the wildtype feline CYB5R3 protein. (B) Protein model of the predicted translation product of transcript mut #1 (XP_044918404.1:p.G76_Q77insERSPDPARVEPG). The 12 inserted amino acids are marked in red. (C) Protein model of the predicted translation product of transcript mut #2 XP_044918404.1:p.(V52Afs*58). We did not experimentally verify whether the predicted proteins were expressed. However, the RT–PCR experiments confirmed that the mut #2 mRNA transcript was present in the affected cat, suggesting that at least a fraction of this aberrant transcript escaped nonsense-mediated mRNA decay.