Table 1.
Venous blood gases, erythrocytic metHb, reduced glutathione concentrations, and erythrocytic CYB5R activity in a methemoglobinemic cat.
| Parameters | At Time of Anesthesia Induction |
After Supplementation * |
After Methylene Blue ** |
After 2 Weeks *** | After ~4 Months *** |
After ~6 Months *** |
Reference Interval **** |
|---|---|---|---|---|---|---|---|
| Hematocrit, % | 29 | 33 | 46 | 45 | 33–45 | ||
| Hemoglobin, g/L | 116 | 121 | 160 | 113–155 | |||
| MCV, fL | 36 | 40 | 39 | 40–48 | |||
| MCHC, g/dL | 40 | 37 | 35 | 33–36 | |||
| Reticulocytes, 106/L | 14,076 | <45,000 | |||||
| O2-Hb,% | 23.7 | 64.5 | 91.1 | 47.1 | 63.4 | 60.4 | >90% # |
| CO-Hb, % | 4.4 | 2.7 | 3.6 | 1.7 | 2.8 | 2.2 | <1% # |
| metHb, % | 49.6 | 39.9 | 3.1 | 32.4 | 33.8 | 35.2 | 0.3–4.0 |
| GSH, µmol/g Hb | 11.9 | 10.1 | 9.4 | 4.8 ± 0.9 | |||
| CYB5R activity, | 0.27 | 0.46 ± 0.10 | 2.07 ± 0.81 | ||||
| IU/g Hb | 13.0 | 22.2 | 100 | ||||
| % |
* Supplement oxygen therapy to reduce methemoglobinemia: Acetylcysteine initially 140 mg/kg then 70 mg/kg, ascorbic acid 10% 30 mg/kg, S-adenosyl methionine (Samyline). ** after a lack of response methylene blue at a dose of 1 mg/kg body weight was administered intravenously. *** No further treatment was needed after initial treatment. **** Reference values from Clinical Laboratory, Vetsuisse Faculty Zurich (Sysmex) or # from [35] Values highlighted in red are out of the reference range. MCV: mean cell volume, MCHC: mean corpuscular hemoglobin concentration. GSH: reduced glutathione.