Table 1.
Stages of the study of the Farnesol effect on M. pachydermatis biofilm formation.
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Action 1 | SDB 100 µL | SDB 100 µL | SDB 100 µL | SDB 100 µL | SDB 100 µL | SDB 100 µL | SDB 100 µL | SDB 100 µL | SDB 100 µL | SDB 100 µL | SDB 100 µL | SDB 100 µL |
| Action 2 | +Farnesol | |||||||||||
| Action 3 | Not titrated | Transfer 100 µL | Transfer 100 µL | Transfer 100 µL | Transfer 100 µL | Transfer 100 µL | Transfer 100 µL | Transfer 100 µL | Transfer 100 µL | Transfer 100 µL | Transfer 100 µL | Transfer 100 µL |
| The concentration of farnesol | Control– no farnesol |
200 μM | 100 μM | 50 μM | 25 μM | 12.5 μM | 6.3 μM | 3.1 μM | 1.6 μM | 0.8 μM | 0.4 μM | 0.2 μM |
| Action 4 | +100 µL of culture | +100 µL of culture | +100 µL of culture | +100 µL of culture | +100 µL of culture | +100 µL of culture | +100 µL of culture | +100 µL of culture | +100 µL of culture | +100 µL of culture | +100 µL of culture | +100 µL of culture |
| Action 5 | wait for 72 h | |||||||||||