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. 2023 Apr 4;12(7):1083. doi: 10.3390/cells12071083

Figure 4.

Figure 4

α-syn PFFs selectively bind to β-isoforms, but not α-isoforms, of NRXs. (A) Representative images showing the binding of biotin-α-syn PFFs (1 µM, monomer equivalent) to COS-7 cells expressing the indicated isoform of extracellularly HA-tagged NRX. S4− and S4+ indicate without and with an insert at splicing site 4, respectively. Surface HA (green) was immunolabelled to verify the expression of these constructs on the COS-7 cell surface. Scale bar: 30 μm. (B) Quantification of the average intensity of bound biotin-α-syn PFFs on COS-7 cells expressing the indicated constructs. Only β-isoforms of NRXs show the significant binding of biotin-α-syn PFFs. α-NRXs have no binding of biotin-α-syn PFFs. Kruskal-Wallis one-way ANOVA, p < 0.0001. **** p < 0.0001 compared with HA-CD4 by Dunn’s multiple comparisons test. N.S., not significant. Data are presented as mean ± SEM. (n = 30 cells for each of three independent experiments).