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. 2023 Mar 29;12(7):1043. doi: 10.3390/cells12071043

Figure 5.

Figure 5

The effects of a four-day RPM exposure on the human squamous non-small-cell lung cancer cell line CRL-5889 according to Dietz and co-workers [196] (A–D). (A) The RPM exposure leads to partial detachment of cells from the bottom of the flask and after 24 h to first aggregations (spheroid formation) that further increase after 96 h. (D, black arrow). However, one part of the cells remains attached to the bottom of the bottle (D, adherent cells (AD), white arrow). These AD cells exhibit an upregulation of the PTEN, RB1, TP53, CDKN2A, and SOX2 gene expression, whose protein-to-protein interactions were shown by STRING analysis (B). In contrast under 1g on 2D cell culture plate the cells stay in a state of near confluence (C). These results are published in [196].