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. 2023 Mar 29;12(7):1040. doi: 10.3390/cells12071040

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of PF-475 on astrocytes and microglia activation after mTBI in hippocampus, cortex and other. Cytosolic fractions of hippocampus tissue were used to evaluate astrocytes and microglia activation trough the expression of GFAP and Iba-1, respectively. Representative blot of Iba-1 is shown (A), see densitometric analysis (A1); representative blot of GFAP is shown (B), see densitometric analysis (B1). Cytosolic fractions of cortex tissue: representative blot of Iba-1 is shown (C), see densitometric analysis (C1); representative blot of GFAP is shown (D), see densitometric analysis (D1). Cytosolic fractions of other tissue: representative blot of Iba-1 is shown (E), see densitometric analysis (E1); representative blot of GFAP is shown (F), see densitometric analysis (F1). Two-way ANOVA test. *** p < 0.001 vs. non mTBI; ### p < 0.001 vs. TBI.