Effects of PF-475 on copper transporters expression after mTBI in hippocampus, cortex and other. Cytosolic fractions of hippocampus tissue were also used to evaluate copper transporters expression, such as CTR1, ATP7A and ATP7B. Representative blots of CTR1, ATP7A and ATP7B are shown (A–C), see densitometric analysis (A1–C1). Cytosolic fractions of cortex tissue: representative blots of CTR1, ATP7A and ATP7B are shown (D–F), see densitometric analysis (D1–F1). Cytosolic fractions of other tissue: representative blots of CTR1, ATP7A and ATP7B are shown (G–I), see densitometric analysis (G1–I1). Two-way ANOVA test. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. non mTBI; # p < 0.05 and ### p < 0.001 vs. TBI.