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. 2023 Mar 24;12(7):1006. doi: 10.3390/cells12071006

Figure 3.

Figure 3

Increased mitochondrial enzymatic activity in the brain and liver of AD mice treated with mitochondrial transfer (A–D). Injected mitochondria are detected in the liver and not in brain (E,F). (A,B) COX/CS activity ratio in the brain and liver of 6-month-old treated mice (four injections). (A) One-way ANOVA showed a significant difference in brain between the groups [f(2, 11) = 8.064, p = 0.007]. LSD post-hoc analysis showed a significantly lower ratio in AD vs. non-AD mice (p = 0.002), with higher ratio in mitochondria-treated vs. untreated AD mice (p = 0.018), reaching the ratio of the non-AD mice. (B) A higher COX/CS ratio was detected in the liver of treated AD mice relative to the untreated AD mice (t-test, p = 0.004), reaching even a higher value than in the liver of the non-AD mice, with a trend showing a reduced ratio in the AD relative to non-AD mice (p = 0.1). (C,D) COX/CS activity ratio in the brain and liver of 12-month-old treated mice (two injections). (C) The treated AD mice showed a higher COX/CS ratio in brain relative to the untreated AD mice (t-test, p = 0.028), reaching the ratio of the non-AD and even exceeding it, with a trend showing a reduced ratio in the AD relative to non-AD mice (p = 0.15). (D) One-way ANOVA showed a trend toward difference in brain between the groups [f(2, 16) = 3.245, p = 0.06]. LSD post-hoc analysis showed a significantly higher COX/CS ratio in the treated compared to the untreated AD mice (p = 0.044), with a reduced ratio in the AD relative to non-AD mice (p = 0.033). (E,F) DsRed signal is detected in the liver and not in the brain of AD mice about 2 h following the injection of DsRed mitochondria. (E) Using IVIS: DsRed signal in the liver of mitochondria-injected (200 mgr/mouse buffer) AD mice but not in their brain (hippocampus region is presented). No signal in the brains and livers of buffer-injected mice. (F) Using anti-RFP Ab fluorescence staining: DsRed signal in the liver of mitochondria-injected (500 mgr/mouse) AD mice but not in their brain. No signal in the brains and livers of buffer-injected mice (* statistically significant; ^ trend).