Figure 5.

PATZ1 overexpression in lung adenocarcinoma cells reduces PD-L1 expression. (A) Western blots using anti-PATZ1 and anti-PD-L1 on total cell extracts of A549 and H1299 cells collected 24 h after transfection with PATZ1-overexpressing plasmid (PATZ1) or empty vector used as control (CTRL). Vinculin was used for normalization. Black dashed lines delineate the boundary between non-contiguous lanes of the same gel. (B) Relative PD-L1 levels were quantified by densitometric analysis using Image J (v1.46r), normalized relative to vinculin levels, and expressed relative to each control, arbitrarily set to 1. a.u., arbitrary units. * p < 0.05; ** p < 0.01. The uncropped blots are shown in Figure S3.