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. 2023 Mar 28;24(7):6322. doi: 10.3390/ijms24076322

Figure 2.

Figure 2

CXCL13 was produced in BMDMs after FMDV infection. (A) The mRNA expressions and the protein levels of CXCL13 in uninfected BMDMs (Mock) and FMDV-infected BMDMs at 24 hpi were quantified by RT-qPCR and ELISA, respectively. (B) CXCL13 (green), F4/80 (red), and nucleus (blue) were co-stained in Mock and FMDV-infected BMDMs at 24 hpi. (C) The FMDV RdRp sequence and FMDV VP1 protein were detected in Mock and FMDV-infected BMDMs at 24 hpi with RT-qPCR and Western blotting, respectively. Upper panels, original magnification ×100 (scale bar = 25 μm). The white box indicated the regions at higher magnifications (scale bar = 7.5 μm). At least 10 fields of immunofluorescence were observed. Significance was detected using unpaired t test. *, p ≤ 0.05; ***, p ≤ 0.001.