Table 1.
Strains | Method | Results | Reference |
---|---|---|---|
S. cellulosum | Inactivation of the epoK gene by TALEN gene knockout system | Epothilone D yield increased to 34.9% and Epothilone B decreased to 34.2% | [33] |
Schlegella brevitalea DSM 7029 | Heterologous expression of different plasmids created by BioBricksTM and SSRTA methods | Enhancement of Epothilone B production to 82 mg/L in 6 days of fermentation | [34] |
S. cellulosum | Optimization of parameters to 30 °C, initial pH = 7.4, speed of 200 r/min, inoculation of 10%, loading amount of 50/250 mL, fermentation 6 days, seed age of 60 h. | Increasing epothilone B production to 39.76 mg/L | [35] |
S. cellulosum | Enhance the epothilone gene cluster with a novel promoter P3 by TALE-TF and CRISPR/dCas9 |
Epothilone B yield increased by 2.89- and 1.53-fold. Epothilone D yield improvement by 1.12- and 2.18-fold | [16] |
S. cellulosum | Fermentation of S. cellulosum modified with plasmids pR6K-Amp-H.a-f-Ptet-H.a-r and pR6K-H.a-f-PBAD-H.a-r | Increasing the Epothilone B production to 93 mg/L | [36] |
Burkholderiales strain DSM 7029 | Electroporation of epothilone gene cluster 56 kb to DSM 7029, plus methylmalonyl-CoA and overexpression of tRNA genes | Increase the yields of epothilones production by 75-fold to 307 μg/L | [37] |
S. cellulosum | Fermentation of immobilized S. cellulosum into porous ceramics | Increasing by 4-Folds the epothilone production to 90.2 mg/L | [38] |