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Relative amplification ratio against the targeted group using DNA isolated from cultures and rpoA-based group-specific primer pairs. A DNA amount of 10 ng was used in each PCR reaction (A) to (G) use primer pairs specific to Groups 1–9, respectively. Samples were run in triplicate in the same plate, and the mean and SD were obtained. ND, not detected. BDL, below the detection limit.
