Fig. 3. Characterization of the CRISPR-Cas13d nanoprodrug and 980 nm light-controlled prodrug reduction. (a) Schematic illustration of the energy migration pathways of Tm³⁺ and Ho³⁺ ions under excitation of an 808 or 980 nm laser. (b) Upconversion luminescence spectra and photograph of URL when irradiated with an 808 or 980 nm laser. (c) TEM images of UCNP@mSiO₂ and UCNP@RB-Pt/RNP@liposome (URL). The distance between the red arrows indicates the thickness of the phospholipid layer. (d) HPLC chromatograms of the solution containing UCNP@RB-Pt with different treatments. UCNP@RB-Pt (50 μg mL⁻¹); GSH (10 mM); 980 nm (0.5 W cm⁻², 5 min). (e) XPS curves of Pt 4f of UCNP@RB-Pt before and after being irradiated with a 980 nm laser, UCNP@RB-Pt (2 mg mL⁻¹); GSH (10 mM); 980 nm (0.5 W cm⁻², 5 min). (f) Schematic illustration of the synthesis of water-soluble URL. (g) ROS level of the solution containing URL NPs with irradiation of an 808 or 980 nm laser. URL (200 μg mL⁻¹); 980/808 nm (0.5 W cm⁻², 5 min). (h) Intracellular GSH depletion under irradiation of an 808 or 980 nm laser. URL (100 μg mL⁻¹); 980/808 nm (0.5 W cm⁻², 5 min). **P < 0.01, and ****P < 0.0001.