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Nitroreductase (NTR)-mediated reduction and activation of NIR-HCy-NO2 (A) and fluorescence enhancement of NIR-HCy-NO2 in the presence of 5 µg/mL NTR and 50 µM NADH over 30 min (B–D). (B) NIR-HCy-NO2 1 in 1× PBS (pH 7.4, 20% (v/v) ACN), (C) NIR-HCy-NO2 2 in 1× PBS (pH 7.4, 5% (v/v) ACN), and (D) NIR-HCy-NO2 3 in 1× PBS (pH 7.4). The emission spectra were recorded using λex = 672 nm.
