Figure 6.

Periostin reprogrammed macrophages partly through integrin αM/p-Erk/Erk signaling. (A,B) Flow cytometric analysis for pro- and anti-inflammatory marker expression in macrophages after periostin stimulation with or without Erk inhibitor. The data were analyzed by one-way ANOVA and Bonferroni post hoc test (n = 3). L-R: group treated with RPMI1640 alone; L-P: group treated with rhPeriostin; L-P-D: group treated with rhPeriostin and DMSO; L-P-U₁: group treated with rhPeriostin and 10 μg/mL U0126; L-P-U₂: group treated with rhPeriostin and 20 μg/mL U0126. (C,D) Western blot analysis of CD163 and phosphorylation of ERK in macrophages after periostin stimulation with or without anti-ITGAM antibodies. IgG isotype control or anti-ITGAM antibodies were added to examine the role of integrin αM in signaling. The data were analyzed by one-way ANOVA and Bonferroni post hoc test (n = 3). * p < 0.05; ** p < 0.01; *** p < 0.001. Error bars represent means ± SD.