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Treatment regime. After initial transthoracic echocardiography, mice received a single intraperitoneal (i.p.) injection of Doxorubicin (20 mg/kg; 55 mg/m2). After 6 days, heart function was re-evaluated using echocardiography. Mice were sacrificed and the effects of Doxorubicin on cardiac PD1/PDL1 signaling were analyzed using flow cytometry and immunohistochemistry.
