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Propolis ethanolic extract (PEE) increased the viability of D-galactose (D-gal)-treated C2C12 cells and attenuated the senescence of C2C12 cells. (A) Effect of PEE (1, 5, 25 µg/mL) on the viability of D-gal-treated C2C12 cells. (B) Senescence-associated β-galactosidase (SA-β-Gal) staining and quantification for C2C12 cells (scale bar = 100 μm). All data are expressed as mean ± SEM (n = 3). ** p < 0.01 vs. Ctrl; ## p < 0.01 vs. D-gal.
