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. 2023 Mar 24;24(7):6137. doi: 10.3390/ijms24076137

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expression analysis of the top four TF family genes was examined using hierarchical clustering in different time-series of chilling and freezing stresses. FPKM values of representative transcript levels of TF members. The standardized expression data was used to generate a heatmap with hierarchical clustering based on the Manhattan correlation with average linkage using the MeV software package. Expression levels of (A) AP2/ERF, (B) bHLH, (C) MYB, (D) C2H2-zinc finger genes under chilling and freezing stresses at 1, 8, and 24 h. The heatmap shows the change in the average log2 (fold-change) of each transcript.