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. 2023 Mar 23;24(7):6021. doi: 10.3390/ijms24076021

Figure 3.

Figure 3

EVO induces cell death and cell cycle arrest through ferroptosis in TCCSUP cells. (A) TCCSUP cells were treated with 100 µM DFO or the vehicle (DMSO) for 1 h, followed by treatment with 15 µM EVO or the vehicle (DMSO) for 24 h. Representative phase-contrast microscopic images of EVO-induced cell death are shown (Scale bar = 100 μm). (B) TCCSUP cells were treated with DFO (100 µM), Z-VAD-FMK (20 µM), necrostatin-1 (Nec-1, 50 µM), or the vehicle (DMSO) for 1 h, followed by treatment with 15 µM EVO or the vehicle (DMSO) for 24 h. Cell viability was analyzed by the MTS assay (n = 8 for each group). (C,D) Cell cycle analysis with PI staining and flow cytometry after EVO treatment for 24 h with or without prior DFO treatment for 1 h. DNA content histograms (C) and quantitation (D) of different cell cycle phases (n = 6 for each group) are shown. Data are mean ± SEM. ** p < 0.01, *** p < 0.001 as compared with the control group.