Figure 5.

EVO inhibits tumor growth and induces MET in NOD-SCID mice bearing TCCSUP tumor xenografts. (A–G) Groups of 14 NOD/SCID mice were inoculated subcutaneously with TCCSUP cells (1 × 10⁶) at day 0, followed by intraperitoneal injection of EVO (20 mg/kg) or PBS for 35 consecutive days. Representative images of gross appearance of tumors excised at day 35 (A). Tumor volumes were measured weekly for 5 weeks and expressed as a percentage of the tumor volume at day 7 in each group (B). Detection (C) and quantitation of E-cadherin (D), N-cadherin (E), vimentin (F), and Snail (G) proteins in day 35 tumor tissues by Western blot analysis (C) and densitometric analysis using the ImageJ software (n = 5 for each group) (D–G). Expression of β-actin served as the loading control. Data are mean ± SEM. * p < 0.05, ** p < 0.01 as compared with the control group.