Figure 1.
Thiosulfinate-enriched Allium sativum extract (TASE) effects on oxidative stress of monocytes from both HV and septic patients. (A) Monocytes from HV (n = 15) and septic patients (n = 7) were labeled with Green CellROX. Mean intensities of fluorescence (MIF) on gated CD14+ monocytes determined by flow cytometry are shown. *, p < 0.05 in unpaired t test. (B) Monocytes from HV (n = 15) and septic patients (n = 7) were stimulated with 10 ng/mL of LPS in the presence or not of 3 µg/mL of TASE for 16 h, then cells were labeled with Green CellROX. MIF on gated CD14+ monocytes determined by flow cytometry are shown. KW, Kruskal–Wallis statistic; #, p < 0.05; ##, p < 0.01 in Dunn’s post hoc test. *, p < 0.05; **, p < 0.01 in paired t test. White dots represent individual HV values and red dots individual sepsis values. Bars express mean ± SD. Left panels show individual paired analyses.