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. 2023 Mar 24;24(7):6160. doi: 10.3390/ijms24076160

Figure 6.

Figure 6

Effect of 1,8-cineole on Keap1, Nrf2, HO1, NQO1, superoxide dismutase (SOD), and catalase (CAT) activity. The upper panel shows (a) Keap1 and (b,c) cytosolic Nrf2 and nuclear Nrf2 protein expression. Representative protein bands were normalized to GAPDH and lamin proteins as the internal controls as shown. Densitometric analysis showed that DSS administration increased Keap1 protein expression while 1,8-cineole did not further increase Keap1 protein expression. Nuclear Nrf2 protein expression was markedly increased by 1,8-cineole treatment compared to the DSS-administered group. (c) DSS administration significantly decreased (d) NQO1, (f) HO1 protein, and (e,g) mRNA expression, while 1,8-cineole treatment prevented these decreases. DSS administration significantly decreased (h) colon tissue SOD and (i) CAT enzyme activity, while 1,8-cineole treatment restored their activities toward the control levels. Data were obtained from n = 4 for protein, n = 6 for mRNA, and n = 8 animals for SOD and CAT enzyme activity; the results are expressed as the means ± SEM. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001, and ns indicates results that were not significant.