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. 2023 Mar 24;24(7):6160. doi: 10.3390/ijms24076160

Figure 8.

Figure 8

Effect of 1,8-cineole on HT-29 cell viability, proinflammatory chemokine mRNA expression, PPARγ expression, and PPARγ promoter assay. (a) 1,8-cineole treatment did not affect HT-29 cell viability up to a 200 μm concentration. (b,c) 1,8-cineole significantly downregulated IL8 and CXCL1 mRNA expression in TNF-α-challenged HT-29 cells. (d,e) 1,8-cineole significantly increased PPARγ protein and mRNA expression. (f,g) PPARγ siRNA significantly downregulated PPARγ protein and mRNA expression. (h) PPARγ siRNA transfection downregulated IL8 mRNA expression, and 1,8-cineole treatment partially prevented this. (i) siRNA transfection did not affect CXCL1 mRNA expression in the presence of 1,8-cineole. (j,k) 1,8-cineole treatment significantly enhanced PPARγ promoter activation time-dependently (12 and 24 h). The PPARγ activator (GW1929) and PPARγ inhibitor (GW9662) worked as expected. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, ****p ≤ 0.0001, and ns indicate results that were not significant.