Figure 4: Activating mTOR mutations are detrimental under glucose and O₂ limiting conditions.

Relative cell number of WT MEFs expressing empty vector (control) or expressing mTOR WT or mTOR mutants treated with A) complete medium or B) medium without glucose. The cell number at indicated days relative to the number at the start of the treatment (d0) is shown. C) Glucose consumed and D) Lactate secreted per million cells per hour. Relative cell number of WT Mefs expressing empty vector (control) or expressing mTOR WT or mTOR mutants treated with E) 0.5% O2 or F) 0.5% O2 and 0.1mM Glucose. This represents the cell number at d3 over d0, where d0 is the number of cells at the start of treatment. Results are representative of three independent experiments performed in triplicate and are expressed as mean ± SD (error bars) of three replicates. Statistical significance is based on one-way ANOVA. p values are as indicated: ns = not significant; * , p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001.