Figure 1.

Digital cytometry analysis validates vascular cells as the overall most predominant cell type sorted by FACS at 3- and 7-days post-injury (n = 2–3). Single-cell gene expression data from spinal cord mouse tissue acquired from the Brain Mouse Atlas were used to build the cell signatures using 20 replicates from 50 cells classified with TaxonomyRank1 [19]. Next, cellular abundance was estimated with the bulk transcriptomic data using the subset of high-confidence genes. Cell signatures and cell type abundance were determined using CIBERSORTx [18].