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. 2023 Apr 1;24(7):6597. doi: 10.3390/ijms24076597

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of low-Na⁺ acclimation on Ncc2 expression in the gills of adult zebrafish. The mRNA expression levels of slc12a10.2 (Ncc2) in the gills were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) after acclimation to low-Na⁺ FW. qRT-PCR data were normalized to rpl13a (N = 7) (a). The protein expression of Ncc2 in the gills acclimated to low-Na⁺ FW was analyzed by Western blot. The blots show the bands with molecular weights corresponding to Ncc2 and GAPDH (approximately 112 kDa and 35 kDa, respectively). The protein expression of Ncc2 was quantified and normalized to GAPDH (N = 6) (b). Values are the mean ± SD. Student’s t-test, * p < 0.05. ns, not significant.