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. 2023 Apr 1;24(7):6597. doi: 10.3390/ijms24076597

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of low-Na⁺ acclimation on the number of NCC ionocytes and Ncc2 expression within individual NCC ionocytes in the gills of adult zebrafish. The cell number of NCC ionocytes and the fluorescence intensity of Ncc2 signals in the gill filaments were analyzed by whole-mount IF after acclimation to low-Na⁺ FW (a). NCC ionocyte number of filaments (N = 12) (b), mean fluorescence intensity of individual NCC ionocytes (N = 531–1017) (c), and the average mean fluorescence intensity of NCC ionocytes of filaments (N = 12) (d) are shown. Values are the mean ± SD or SEM. Student’s t-test or Mann–Whitney U test, *** p < 0.001, **** p < 0.0001. Scale bar, 50 μm.