Figure 2.

Impact of PD-L1 knockout on proliferation rate and colony growth of MDA-MB-231. (A) Control- and PD-L1 knockout cells were seeded into six-well plates for 1, 2, 3, and 4 days. The cells proliferation was determined using a cell counter as described in the Materials and Methods. (B,C) The control and knockout cells were seeded and kept to form colonies for 3 weeks after which the colonies were fixed, stained, and counted, as described in the Materials and Methods. Experiments were repeated three times. Shapes/Columns represent mean; bars represent S.E.M. ** p < 0.01, *** p < 0.001, **** p < 0.0001.