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. 2023 Mar 29;24(7):6420. doi: 10.3390/ijms24076420

Figure 5.

Figure 5

PD-L1 knockout significantly decreases MDA-MB-231 invasion in vitro. (A) Using Boyden Matrigel invasion chamber assay, control and PD-L1 knockout cells were seeded in the upper chamber for 24 h. The invading cells into the lower chamber were determined using CellTiter-Glo Luminescent. (B,C) Using Oris Matrigel invasion assay, invasion of the control and PD-L1 knockout cells into the detection zone was monitored for 24 and 48 h. Experiments were repeated at least 3 times. Columns are means; bars are S.E.M. ** p < 0.01. *** p < 0.001. **** p < 0.0001.