Figure 3.

OxPhos subunits and UCP2 expression in lymphoblasts and fibroblasts mutated for the FANC-A gene. Western blot (WB) signals of OxPhos subunits expression in lymphoblast (A) and fibroblast (B) cell lines with and without FANC-A mutation. Protein expression levels are normalized on the housekeeping signal (Actin) revealed on the same membrane: ND1 (Complex I), SDHB (Complex II), MTCO2 (Complex III), and ATP synthase β subunit; WB signal of uncoupling protein 2 (UCP2) expression. Data in histograms are reported as mean ± SD, and each panel is representative of at least three independent experiments. Statistical significance was tested opportunely with an unpaired t-test; ** represents a significant difference for p < 0.01 between FA and FAcorr cells.