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. 2023 Apr 5;24(7):6763. doi: 10.3390/ijms24076763

Figure 1.

Figure 1

CA venom induces loss of substrate contact and decreased viability in HUVEC. (A) Phase contrast images of HUVEC monolayers stimulated with 10 or 100 µg/ml CA venom for 3 h. MTS assay demonstrates significant decrease in HUVEC viability after 3 h stimulation with 10 or 100 µg/ml CA venom. * p < 0.05 vs. control ** p < 0.01 vs. non-stimulated control (n = 3). (B) Multi-Western blotting of focal contact and apoptotic proteins. HUVEC monolayers stimulated with CA venom (10 µg/mL) for 3 h. Focal contact proteins indicated by white arrow and apoptotic proteins (black arrows). Control included non-stimulated lysates. (C) Conventional Western blotting of Hic-5 and β-tubulin loading control.